The Analytical and Translational Genomics Resource primarily provides next generation sequencing technologies such as RNA-seq, targeted gene panel sequencing, exome sequencing and epigenetics assays such as ChIP-seq and RRBS, coupled with expert bioinformatics analysis. Affymetrix microarray and real-time PCR services are also available. The ATG Shared Resource is available for use by all faculty at UNM and its affiliates, and all investigators are encouraged to contact us to find out how we can help with their research.
Ion S5XL Next-Generation Sequencing: The powerful Life Technologies Ion S5XL semiconductor sequencing instrument is ideal for next-generation sequencing assays including exome sequencing (all the proton-coding exons, about 2% of the genome), gene expression (RNA-seq) assays, epigenetics assays (ChIP-seq) and targeted sequencing (Ion Ampliseq Comprehensive Cancer Panel) of cancer-relevant genes, even from FFPE samples.
Expert Bioinformatics Data Analysis: ATG Shared Resource staff use sophisticated data analysis methods to analyze gene expression and genotyping data and strive to provide our users with publication-quality figures for their manuscripts or grant applications. We use R/Bioconductor and commercial software packages to explore the large and complicated data sets generated by genomics methods.
10X Genomics Single Cell: The ATG shared resource has a complete 10X Genomics Chromium Controller that allows users to bring ATG Single Cell Suspensions and Single Cell Libraries are produced. There are several assays available through 10X genomics that ATG is able to provide to our users.
Scott A. Ness, PhD
Professor, Internal Medicine: Molecular Medicine
Associate Director, UNM Cancer Center
Office: CRF 121A; Tel: (505) 272-9883
Hours | Location |
Monday - Friday 8:00 AM - 5 PM |
Cancer Research Facility Rooms: 118, and 124 |
Fixed Cost Services |
► 10X Genomics (Single Cell) (1) | |||
Name | Description | Price | |
---|---|---|---|
10x 3' or 5' Library Preparation Per Library |
This to prepare a single cell suspension sample into a 3'or 5'single cell library for Illumina Sequencing. Includes basic set up, labor and instrument fee for the 10x Genomics instrument. |
Inquire | |
► Data Analysis (2) | |||
Name | Description | Price | |
AWS Cloud Computing (per project / period) |
Internal (UNM Member)
$158.00
each
Internal (CC Member) $158.00 each External (Non-Profit) $237.00 each Corporate $237.00 each |
||
Bioinformatics Analysis (~20 hr) |
Includes initial consultation, quality controls, workflow design, data processing, storage and support for up to 1 year for this project only. Some projects will require more than one of these charges. |
Internal (UNM Member)
$2,047.00
each
Internal (CC Member) $2,047.00 each External (Non-Profit) $3,070.50 each Corporate $3,070.50 each |
|
► Illumina Sequencing (4) | |||
Name | Description | Price | |
FedEx Shipping Fee |
This Fee is to have your samples shipped out to be Sequenced on an Illumina Platform. |
Inquire | |
Illumina Sequencing Per Lane at Univ of CO |
This is sequencing your sample on the Illumina Platform. SP, S1, S2 or S4 whole flow cell. |
Inquire | |
Low Pass Sequencing |
This is running a Low Pass Sequencing on your sample, resulting in 40 million reads per sample. If the results look good,a deeper sequencing will be ran. |
Inquire | |
Sequencing 10 million Reads /20 Million PE Reads |
This is sequencing your sample on the Illumina Platform. |
Inquire | |
► Instrument Annual Fee (3) | |||
Name | Description | Price | |
Agilent BioAnalyzer Annual Fee |
Per lab/per year |
Inquire | |
Nanodrop Spectrophotometer Annual Fee |
Per lab/Per year |
Inquire | |
Qiagen Octo Dissociator Annual Fee |
per lab/per year |
Inquire | |
► Ion Proton (2) | |||
Name | Description | Price | |
Ion S5 and Ion Chef Labor, Set-up and Data Charges (per chip) |
This includes the labor to set up and run the machines, the instrument fee (pays our service contract) and the bioinformatics charge (running plugins, moving data, storing data, ect.) One chip is usually 4 samples. |
Inquire | |
S5 540 Chip and HI-Q Chef Reagents |
This is for the price of the Chip and the Chef Reagents. |
Inquire | |
► Lab Services (4) | |||
Name | Description | Price | |
Agilent Bioanalyzer Chip, DNA or RNA (each) |
Internal (UNM Member)
$80.00
each
Internal (CC Member) $80.00 each External (Non-Profit) $120.00 each Corporate $120.00 each |
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Laboratory Labor Per Hour |
Expert ATG Staff labor charge |
Inquire | |
Reagents and Disposables | Inquire | ||
Sample/Library Prep Labor, w/o Library Kits (per 4 libraries) |
This is the cost Per Project to make DNA/RNA and prepare the libraries by ATG Staff |
Inquire |